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L7136 Sigma

Lipopolysaccharides from Salmonella typhosa

purified by trichloroacetic acid extraction

Synonym: LPS

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates H-L, Complex Lipids,
form   lyophilized powder
purified by   trichloroacetic acid extraction
impurities   1-10% Protein (Lowry)
storage temp.   2-8°C

Description

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

General description

This product is TCA extracted from Salmonella typhosa. The source strain is ATCC 10749.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Lipopolysaccharides (LPS)

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane...
Keywords: Adsorption, Antibiotics, Cell culture, Cell disruption, Chromatography, Culture media, Filtration, Immunology, Ion Exchange, Metabolism, Positron Emission Tomography, Purification, Size-exclusion chromatography, Solvents

Peer-Reviewed Papers
15

References

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