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L7391 Sigma

Monoclonal Anti-LIS1 antibody produced in mouse

~2 mg/mL, clone LIS1-338, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurobiology,
species reactivity   bovine, chicken, human, rat, mouse, zebrafish
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 0.5-1 μg/mL using a rat brain cytosol preparation
clone   LIS1-338, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 46 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PAFAH1B1(5048)
mouse ... Pafah1b1(18472)
rat ... Pafah1b1(83572)
biological source   mouse
InChI Key   ZQTAMPRZFOOEEP-AVQMFFATSA-N
conjugate   unconjugated

Description

Immunogen

recombinant human LIS1 protein.

General description

The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa).

Application

Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.

Biochem/physiol Actions

The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.

Price and Availability


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