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L7395 Sigma

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

Synonym: Linoleate:oxygen oxidoreductase, Lipoxygenase

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Properties

Related Categories 1.13.x.x Acting on single donors with incorporation of molecular oxygen, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
type   Type I-B
form   lyophilized powder
mol wt   mol wt 108 kDa (two 54 kDa subunits)
shipped in   wet ice
storage temp.   −20°C

Description

Application

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Packaging

15000000, 75000000, 150000000 units in poly bottle

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Contains stabilizer and NaCl

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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