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L7525 Sigma

L-Lactic Dehydrogenase from porcine heart

ammonium sulfate suspension, ≥200 units/mg protein

Synonym: (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,
form   ammonium sulfate suspension
foreign activity   glutamic-oxalacetic transaminase ≤0.1%
  malic dehydrogenase, glutamic-pyruvic transaminase and pyruvate kinase ≤0.06%
storage temp.   2-8°C
Gene Information   pig ... LDHA(407245)

Description

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Suspension in ammonium sulfate and 0.1 M potassium phosphate, pH 7.0

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Protocols

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

To standardize a procedure for the assay of L-Lactic Dehydrogenase for all sources from heart muscle.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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