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L7533 Sigma

LB Broth with agar (Lennox)

EZMix powder microbial growth medium

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

15g/L Agar
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl

Features and Benefits

Lennox EZMix powder with agar provides:
• A budget-friendly alternative to pre-poured plates
• Granulated, dust-free format for safer handling and faster mixing
• Convenient small package to eliminate weighing
• Easy scale-up using larger package sizes
• Standard formulation

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Packaging

1 kg in poly bottle

Reconstitution

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Legal Information

EZMix is a trademark of Sigma-Aldrich Co. LLC

Zeocin is a registered trademark of Cayla Sarl

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Competent Cells

Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment. Once it enters the cytoplasm, the genetic material may be degraded by nucleases i...
Keywords: Antibiotics, Cloning, Detergents, Electrophoresis, Gel electrophoresis, Genetic, Molecular biology, Peptide synthesis, transformation

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Protocols

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transformation Protocols

From our library of Protocols, Sigma-Aldrich presents Transformation Protocols
Keywords: Antibiotics, Detergents, Electrophoresis, Gel electrophoresis, Mass spectrometry, Peptide synthesis, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

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Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents ac...
Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins, transformation

Peer-Reviewed Papers
15

References

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