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L7543 Sigma

Anti-LC3B antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Autophagy-related protein LC3 B, Anti-Autophagy-related ubiquitin-like modifier LC3 B, Anti-Microtubule-associated proteins 1A/B light chain 3B precursor, Anti-MAP1 light chain 3-like protein 2, Anti-MAP1A/1B light chain 3 B, Anti-MAP1A/MAP1B LC3 B

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology, Antibodies for Cell Cycle,
species reactivity   human, rat, mouse
application(s)   indirect immunofluorescence: 5-10 μg/mL using human HeLa cells
  western blot: 1-2 μg/mL using whole extracts of rat and mouse brain
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 16-18 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAP1LC3B(81631)
mouse ... Map1lc3b(67443)
rat ... Map1lc3b(64862)
biological source   rabbit
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human LC3B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence differs by one amino acid in rat and mouse.

General description

The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

By immunoblotting, the antibody recognizes human, rat, and mouse LC3B-I and LC3B-II (~18 kDa and ~16 kDa, respectively).

Application

Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infecti...
Karyn Huryn-Selvar1, Haizhen Liu1, Janet Anderl1, Jun Ma1, Mark Santos1, Matthew Hsu1, and Luke Armstrong1

1 EMD Millipore, 28820 Single Oak Drive, Temecula, CA, USA 92590
Keywords: Autophagy, Cancer, Diseases, Flow cytometry, Fluorescent microscopy, Gene expression, Immunofluorescence, Microscopy, Tagged proteins, Transduction, Transfection

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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