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L7647 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

Type VI, lyophilized powder

DOWNLOAD MSDS (PDF)

Synonym: Con A

Properties

Related Categories Bioactive Small Molecules, CI-CY, Cell Biology, Cell Signaling and Neuroscience, Concanavalin A (Con A),
type   Type VI
form   lyophilized powder
potency   <20 μg per mL agglutination activity
impurities   Carbohydrate, essentially free
  salt, essentially free
solubility   PBS: soluble4 % (w/v)
storage temp.   −20°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Preparation Note

Highly purified by specific affinity chromatography. Purified by modification of the method of Matsumoto.1

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