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L7651 Sigma

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, for molecular biology

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Lysozyme hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine, a polysaccharide backbone of peptidoglycans in the cell wall structure of many microorganisms. This is particularly useful for lysing Gram-positive and Gram-negative bacteria for subsequent nucleic acid extraction.

Features and Benefits

• Highly purified by repeated crystallization and dialysis
• Each lot is use-tested for isolation of plasmid DNA from E. coli

Preparation Note

3× crystallized

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized powder, essentially salt-free

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
OL5989000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How can I reconstitute Lysozyme from chicken egg white, Product L7651?
For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/mL) in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/mL) yielding a clear to slightly hazy colorless solution. This information can be found in the product datasheet at the following link: http://www2.sigmaaldrich.com/sigma/datasheet/l7651dat.pdf
How stable are solutions of Lysozyme from chicken egg white, Product L7651?
Aqueous solutions should retain activity for at least one month when stored between 2–8 ºC. This information can be found in the product datasheet at the following link: http://www2.sigmaaldrich.com/sigma/datasheet/l7651dat.pdf
Is Lysozyme from chicken egg white, Product L7651, suitable for bacterial lysis prior to DNA purification?
Each lot is verified to be suitable for use as a lysing agent in purifying plasmid DNA from E. coli. You can also use this product in combination with the GenElute™ Bacterial Genomic DNA Kit (Product No. NA2100) to purify genomic DNA from Gram-positive bacteria.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

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Peer-Reviewed Papers
15

References

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