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L7886 Sigma

Lectin from Ricinus communis (castor bean) Agglutinin RCA120

buffered aqueous solution

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Properties

Related Categories Ricinus communis (Castor bean), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   buffered aqueous solution
potency   <4 μg/mL agglutination activity
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

Ricinus communis agglutinin (RCA) occurs in two forms designated RCA60 and RCA120 according to their mol. wt. of approx. 60,000 and 120,000 respectively. Neither RCA60 nor RCA120 is blood group specific. RCA60, also referred to as RCAII, Ricin D or RCL III is extremely toxic, inhibits protein synthesis and has an affinity for N-acetyl-D-galactosamine. RCA120, also referred to as RCAI or RCL (I + II), is an agglutinin and has an affinity for terminal β-D-galactosyl residues.
Conjugates are prepared from affinity purified agglutinin RCA120.

Preparation Note

Purified by affinity chromatography using the method of Lin and Li.

Physical form

Solution in 0.005 M sodium phosphate and 0.2 M sodium chloride, pH 7.2, containing 0.1% sodium azide

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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