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L8402 Sigma

Lysozyme from human neutrophils

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... LYZ(4069)

Description

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This procedure may be used for Lysozyme products. The enzymatic rate determination (A450, Light path = 1 cm) is based on the following reaction:

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Peer-Reviewed Papers
15

References

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