Luciferase from Vibrio fischeri (Photobacterium f) lyophilized powder

Properties

Related Categories	1.14.x.x Acting on paired donors with incorporation or reduction of molecular oxygen, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents, Chemiluminescent compounds, Diagnostic and Analytical Enzymes, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, General Metabolic Enzymes I-Z, Luciferase, Luminescent Compounds/Detection, Metabolomics, Purified Enzymes More...
form	lyophilized powder
composition	Protein, ~40% biuret
storage temp.	−20°C

Description

Features and Benefits

Partially purified, soluble extracts containing FMN-dependent luciferase and NADH- and NADPH-dependent FMN reductases. Produces light in a system containing FMN, NADH or NADPH, and n-decyl aldehyde.

Other Notes

ATCC No. 7744 balance primarily buffer salts and stabilizer.

Physical form

Partially purified lyophilized powder

Application

Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. ¹ It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species. ²