|Related Categories||1.14.x.x Acting on paired donors with incorporation or reduction of molecular oxygen, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,|
|composition||Protein, ~40% biuret|
Partially purified, soluble extracts containing FMN-dependent luciferase and NADH- and NADPH-dependent FMN reductases. Produces light in a system containing FMN, NADH or NADPH, and n-decyl aldehyde.
ATCC No. 7744 balance primarily buffer salts and stabilizer.
Partially purified lyophilized powder
Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. 1 It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species. 2
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Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Rapid detection of tetracyclines and their 4-epimer derivatives from poultry meat with bioluminescent biosensor bacteria. Virolainen NE, Pikkemaat MG, Elferink JW, et al. J. Agric. Food Chem. 56(23), 11065-70, (2008)
Effect of pH, EDTA, and anions on heavy metal toxicity toward a bioluminescent cyanobacterial bioreporter. Rodea-Palomares I, González-García C, Leganés F, et al. Arch. Environ. Contam. Toxicol. 57(3), 477-87, (2009)
Spatiotemporal progression of localized bacterial peritonitis before and after open abdomen lavage monitored by in vivo bioluminescent imaging. Sharma PK, Engels E, Van Oeveren W, et al. Surgery 147(1), 89-97, (2010)
Application of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples. Pikkemaat MG, Rapallini ML, Karp MT, et al. Food Addit. Contam. Part A. Chem. Anal. Control. Expo. Risk Assess. 27(8), 1112-7, (2010)
Comparison of human optimized bacterial luciferase, firefly luciferase, and green fluorescent protein for continuous imaging of cell culture and animal models. Close DM, Hahn RE, Patterson SS, et al. J. Biomed. Opt. 16(4), 047003, (2011)
[Effects of the IbpAB AND ClpA chaperones on DnaKJE-dependent refolding of bacterial luciferases in Escherichia coli cells]. Mel'kina OE, Kotova VIu, Manukhov IV, et al. Mol. Biol. (Mosk.) 45(3), 524-8, (2011)
Stereochemical studies of the type II isopentenyl diphosphate-dimethylallyl diphosphate isomerase implicate the FMN coenzyme in substrate protonation. Calveras J, Thibodeaux CJ, Mansoorabadi SO, et al. ChemBioChem. 13(1), 42-6, (2012)
Differential analysis of bactericidal systems of blood serum with recombinant luminescent Escherichia coli and Bacillus subtilis strains. Deryabin DG, Karimov IF, Manukhov IV, et al. Bull. Exp. Biol. Med. 154(1), 59-63, (2012)
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