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L8629 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Erythroagglutinin PHA-E

Synonym: Phaseolus vulgaris agglutinin, PHA

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Properties

Related Categories Phaseolus vulgaris (Red kidney bean), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   salt-free, lyophilized powder
potency   <10 μg per mL agglutination activity ((with erythryocytes))
composition   Protein, ≥80% biuret
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

5 mg in glass bottle

Preparation Note

Purified by a modification of the method of Leavitt.

Quality

Tested for leucoagglutination.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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