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L8754 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

Synonym: Phaseolus vulgaris agglutinin, PHA

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Properties

Related Categories Phaseolus vulgaris (Red kidney bean), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   lyophilized powder
potency   ≤62.5 μg per mL agglutination activity
  ≤10 μg/mL mitogenic activity
impurities   salt, free
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

25, 50 mg in poly bottle

5 mg in glass bottle

Application

Lectin from Phaseolus vulgaris (red kidney bean) is used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.

General description

Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.

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Laboratory Gloves

Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TJ3470000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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