|Related Categories||Phaseolus vulgaris (Red kidney bean), Bioactive Small Molecules, Cell Biology, Glycan Recognizing Proteins, Glycobiology,|
|potency||<62.5 μg per mL agglutination activity|
|<10 μg/mL mitogenic activity|
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
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Leucoagglutinin PHA-L, lyophilized powder
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Phytohemagglutinin PHA-M, lyophilized powder
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