Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder

Properties

Related Categories	Phaseolus vulgaris (Red kidney bean), Bioactive Small Molecules, Cell Biology, Glycan Recognizing Proteins, Glycobiology, L, Lectins, Post-Translational Modification, Proteomics More...
form	lyophilized powder
potency	<62.5 μg per mL agglutination activity
	<10 μg/mL mitogenic activity
impurities	salt, free
storage temp.	2-8°C

Description

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.^1,²
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.³ PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.⁴ PHA-M is the mucoprotein form.⁴ Conjugates are prepared from the corresponding purified lectins.

Price and Availability

Suggested Laboratory Gloves

This substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

for splash & immersion protection