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L8777 Sigma

Lectin from Phytolacca americana (pokeweed)

lyophilized powder, BioReagent, suitable for cell culture

Synonym: PWM, Pokeweed mitogen

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Immune Cell Signaling and Blood, Immune System Regulation,
sterility   aseptically filled
product line   BioReagent
form   lyophilized powder
potency   ≤0.3 μg/mL mitogenic activity
solubility   PBS: soluble1 mg/mL
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Analysis Note

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Biochem/physiol Actions

PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.

Packaging

5, 10 mg in glass bottle

Quality

Partially purified TCA precipitate.
Essentially salt-free.

Application

Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).

General description

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
PZ3540000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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