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L8902 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Synonym: Phaseolus vulgaris agglutinin, PHA

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Description

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

25, 100 mg in glass bottle

Application

Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.

Price and Availability


Amicon Pro Purification System

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TJ3470000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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L4144 Lectin from Phaseolus vulgaris (red kidney bean), Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

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