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L9017 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder

Synonym: Phaseolus vulgaris agglutinin, PHA

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Properties

Related Categories Phaseolus vulgaris (Red kidney bean), Cell Biology, Cell Signaling and Neuroscience, Glycan Recognizing Proteins, Glycobiology,
product line   BioXtra
form   lyophilized powder
potency   ≤17 μg per mL agglutination activity
purified by   affinity chromatography
impurities   salt, essentially free
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

1, 5, 10 mg in glass bottle

Quality

Mitogenic activity at ≤10 μg per mL.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TJ3470000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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