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L9017 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder

Synonym: Phaseolus vulgaris agglutinin, PHA

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Properties

Related Categories Phaseolus vulgaris (Red kidney bean), Cell Biology, Cell Signaling and Neuroscience, Glycan Recognizing Proteins, Glycobiology,
product line   BioXtra
form   lyophilized powder
potency   ≤17 μg per mL agglutination activity
purified by   affinity chromatography
impurities   salt, essentially free
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

1, 5, 10 mg in glass bottle

Quality

Mitogenic activity at ≤10 μg per mL.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TJ3470000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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