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L9031 Sigma

Lipase from Mucor miehei

lyophilized powder, ≥4,000 units/mg solid (using olive oil)

Synonym: Triacylglycerol acylhydrolase, Triacylglycerol lipase

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Description

Analysis Note

Protein determined by biuret.

Biochem/physiol Actions

Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

General description

Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Application

Lipase from Mucor miehei can be used to drive the synthesis of flavor esters.

Lipase from Mucor miehei has been used in a study to assess the nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. It has also been used in a study to investigate the hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TO9776500
Protocols & Articles

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