• USA Home
  • L9132 - Lectin from Phaseolus vulgaris (red kidney bean)

L9132 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, cell culture tested, lyophilized powder

Synonym: Phaseolus vulgaris agglutinin, PHA



form   lyophilized powder
potency   ≤16 μg per mL agglutination activity (with erythrocytes)
  ≤10 μg per mL mitogenic activity
impurities   salt, free
solubility   PBS: soluble5 mg/mL
suitability   cell culture tested
storage temp.   2-8°C


Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25°C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles
Related Products

comparable product

Product #


Add to Cart

L9017 Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder

replaced by

Product #


Add to Cart

L1668 Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?