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L9132 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, cell culture tested, lyophilized powder

Synonym: Phaseolus vulgaris agglutinin, PHA

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Properties

form   lyophilized powder
potency   ≤16 μg per mL agglutination activity (with erythrocytes)
  ≤10 μg per mL mitogenic activity
impurities   salt, free
solubility   PBS: soluble5 mg/mL
suitability   cell culture tested
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25°C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TJ3470000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
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L9017 Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder

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