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L9152 Sigma

Anti-Leukemia Inhibitory Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-LIF

Description

Immunogen

purified, E. coli-derived, recombinant mouse LIF.

General description

Leukemia inhibitory factor (LIF) is a pleotrophic glycoprotein that belongs to the IL6 family of cytokines. It is secreted by many cell types such as, fibroblasts, macrophages, T cells, monocytes, liver cells, osteoblasts and cells of endometrium. LIF interacts with a heterodimeric receptor composed of two subunits, gp130 and gp190.A variety of signal transduction pathways are triggered in response to LIF including ERK/MAPK, JAK1, PI3K, STAT3, Jun-cFos and NF-κB. The various functions of LIF include proliferation of adult human T cells, maintenance of pluripotency of embryonic stem cells and inhibition of leukemic ell differentiation. Reports also indicate the important role of LIF in lactation, tissue regeneration after brain and spinal cord injury, muscle regeneration and motor neuron functions.
Anti-Leukemia Inhibitory Factor recognizes mouse leukemia inhibitory factor. The antibody shows <10% cross reactivity with recombinant human LIF.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Specificity

The antibody neutralizes the biological activity of recombinant mouse LIF and also neutralizes the biologicl activity of recombinant human LIF at a 10-fold higher IgG concentration. Based on ELISA and immunoblotting, this antibody shows <10% cross-reactiity with recombinant human LIF.

Preparation Note

Purified using protein G.

Application

Anti-Leukemia Inhibitory Factor antibody may be used for immunoblotting a concentration of 1-2 μg/ml. For immunohistochemistry, a working dilution of 10 μg/ml is recommended for 5-15 microm thick cryostat sections; for free floating sections, a working concentration of 1-2 μg/ml may be used. The antibody is also suitable for neutralization reactions.

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