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L9393 Sigma

Anti-Laminin antibody produced in rabbit

0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
species reactivity   animal, human
application(s)   dot blot: 1:1,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues
  microarray: suitable
clone   polyclonal
concentration   0.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... LAMA1(284217)
mouse ... Lama1(16772)
biological source   rabbit
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

General description

Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Specificity

Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources.

The antibody shows no cross-reaction with collagen type IV, fibronectin or chondroitin sulfate types A, B, and C using a dot blot immunoassay.

Application

Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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