Synonym: Luciferase firefly
|Related Categories||1.13.x.x Acting on single donors with incorporation of molecular oxygen, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,|
|mol wt||mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)|
Protein, 10-35% E|
|measuring range||≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)|
|foreign activity||ATPase ≤5 nanomolar units/mg protein|
|Nucleoside diphosphokinase ≤20 nanomolar units/mg protein|
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units.
Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.
Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. 2 The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light.
Sold on the basis of protein content
Chromatographically prepared and crystallized.
One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C.
Lyophilized powder approximately 20% protein; balance is primarily NaCl, HEPES buffer salts, and carbohydrate.
The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. Less than or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
This enzyme has wide range of applications in biotechnology and development of biosensors. 2 Luciferase can be used to detect trace amounts of ATP and is one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. Less than or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase. This enzyme has been used in a study to identify the different characteristics of reporter genes in whole-cell bacterial sensors. Luciferase from Photinus pyralis has also been used in a study to develop a novel bioluminogenic assay for α-chymotrypsin.
Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging. 1
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The chemiluminescence of firefly luciferin. A model for the bioluminescent reaction and identification of the product excited state. Hopkins TA, Seliger HH, White EH, et al. J. Am. Chem. Soc. 89(26), 7148-50, (1967)
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Determination of bacterial content in fluids,. Chappelle, E.W., et al. Meth. Enzymol. 57, 65-72, (1978)
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