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L9514 Sigma

Ricin A chain from Ricinus communis (castor bean)

buffered aqueous glycerol solution

Properties

Related Categories Ricinus communis (Castor bean), Bioactive Small Molecules, Cell Biology, Glycan Recognizing Proteins, Glycobiology,
form   buffered aqueous glycerol solution
potency   ≥250 μg/mL per mL agglutination activity
composition   Protein, ≥0.3 mg/mL Lowry-TCA
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.

Biochem/physiol Actions

Ricin A chain inactivates the 60 S ribosomal subunit of eukaryotic cells, inhibiting protein synthesis. This toxic activity is independent of the presence of the B chain of ricin.

Other Notes

Ricinus communis toxin (also named RCA60, RCAII, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.

Preparation Note

Purified by affinity chromatography.

Quality

Electrophoretically pure (SDS-PAGE)

Physical form

Solution in 40% glycerol containing 10 mM phosphate, pH 6.0, 0.15 M NaCl, 10 mM galactose and 0.5 mM dithioerythritol

Price and Availability


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