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L9514 Sigma

Ricin A chain from Ricinus communis (castor bean)

buffered aqueous glycerol solution

Properties

Related Categories Ricinus communis (Castor bean), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   buffered aqueous glycerol solution
potency   ≥250 μg/mL per mL agglutination activity
composition   Protein, ≥0.3 mg/mL Lowry-TCA
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.

Biochem/physiol Actions

Ricin A chain inactivates the 60 S ribosomal subunit of eukaryotic cells, inhibiting protein synthesis. This toxic activity is independent of the presence of the B chain of ricin.

Other Notes

Ricinus communis toxin (also named RCA60, RCAII, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.

Preparation Note

Purified by affinity chromatography.

Quality

Electrophoretically pure (SDS-PAGE)

Physical form

Solution in 40% glycerol containing 10 mM phosphate, pH 6.0, 0.15 M NaCl, 10 mM galactose and 0.5 mM dithioerythritol

Price and Availability

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