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L9516 Sigma

Lipopolysaccharides (rough strains) from Salmonella enterica serotype typhimurium SL1181

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates H-L, Complex Lipids,
form   lyophilized powder
impurities   ≤5% Protein (Lowry)
storage temp.   2-8°C

Description

Packaging

5 mg in glass bottle

Preparation Note

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

General description

This product is phenol:chloroform:petroleum ether extracted from Salmonella typhimurium strain SL1181. The source strain is from a private collection. This LPS has been used as a negative control for antibody binding to LPS.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Protocols

Lipopolysaccharides

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane...
Keywords: Adsorption, Antibiotics, Cell culture, Cell disruption, Chromatography, Culture media, Filtration, Immunology, Ion Exchange, Metabolism, Positron Emission Tomography, Purification, Size-exclusion chromatography, Solvents

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