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L9895 Sigma

Lectin from Pseudomonas aeruginosa

lyophilized powder

Synonym: PA-I

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Properties

Related Categories Pseudomonas aeruginosa, Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   lyophilized powder
potency   <15 μg/mL per mL agglutination activity
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 7.0. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group A erythrocytes after one hr incubation at 25 °C.

Biochem/physiol Actions

PA-I has a major affinity for D-galactose and its derivatives. PA-I agglutinates all types of human and animal erythrocytes and interacts also with unicellular protozoa, algae and bacteria. PA-I induces mitogenic stimulation in cultured human lymphocytes pretreated with neuraminidase.

Packaging

1, 2 mg in glass bottle

Package size based on protein content. Vial content based on protein by biuret.

Preparation Note

Purified by affinity chromatography using a modification of Gilboa-Garber.1

Quality

Highly purified.

Physical form

Lyophilized powder containing approx. 80% protein (biuret), balance primarily sodium citrate, plus traces of calcium chloride, magnesium chloride and manganese chloride

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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