LIG1 Sigma

DNA Ligation Kit

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Properties

Related Categories Cloning and Expression, Ligation Kits, Molecular Biology More...
usage   1 kit sufficient for 150 ligation reactions
shipped in   wet ice
storage temp.   −20°C

Description

Other Notes

Contains all of the reagents necessary to perform DNA ligation reactions. Blunt-end ligations are less efficient than sticky-end ligations. Polyethylene glycol (PEG 8000) facilitates blunt-end ligations.

Application

The DNA Ligation Kit is used to join fragments of DNA during basic biotechnology experiments such as protein expression, mutagenesis, gene anyalysis and structure-function relationships. The kit contains all of the reagents necessary to have consistent ligations (buffer, ATP, T4 DNA ligase, Polyethylene Glycol 8000).

Price and Availability

Kit component only

Description

Product #

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10× ligation buffer 300 μL    
24% PEG 8000 1.5 mL    
10 mM ATP 3×100 μL    
Control DNA pBR322/Hae III digest 50 μL    

Standard component

Description

Product #

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T4 DNA ligase 300 units    
Water 1.5 mL W4502

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