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LIG2 Sigma

QuickLink DNA Ligation Kit

joins blunt-end and sticky-end DNA fragments

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Properties

Related Categories Cell Biology, Circuit Design and Metabolic Pathway Engineering, Cloning, Directional Cloning System, Modifying Enzymes,
grade   for molecular biology
form   liquid
usage   1 kit sufficient for 50 ligation reactions
shipped in   dry ice
storage temp.   −70°C

Description

Components

Sufficient for 50 reactions:
• 500uL 2X Ligation Buffer A (L9537)
• 100uL 5X Ligation Buffer B (L9662)
• 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT

Features and Benefits

• Fast 5 minutes ligation
• High ligation efficiency
• Room temperature reactions – no cooling required
• Perform bacterial transformation with the reaction mixture

General description

Sigma’s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup.

Principle

One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

Application

The QuickLink DNA Ligation Kit is suitable for:
• Joining blunt or cohesive-end fragments of DNA into a cloning vector
• Recircularization of linear DNA
• Formation of concatamers
• dsDNA nick repair

Legal Information

QuickLink is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Protocols

QuickLink™ DNA Ligation Kit

Product Description Components Reagents Required But Not Provided Precautions and Disclaimer Storage/Stability Procedure Troubleshooting Guide Materials References
Keywords: AGE, Antibiotics, Cloning, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Polymerase chain reaction, Precipitation, Purification, transformation

Peer-Reviewed Papers
15

References

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