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M0268 Sigma

Minimum Essential Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym: MEM

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Properties

Related Categories Cell Culture, Classic Media and Salts, Minimum Essential Medium Eagle (MEM), Powder Media
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: yes
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Other Notes

Essentially the same as the original formula, except it is provided as a powder.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

Minimum Essential Medium Eagle - MEM Media

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain sub...
Keywords: Addition reactions, Adhesion, Cell culture, Cloning, Culture media, Eliminations, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

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