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Manual of Immunological Methods

  •  ISBN-10 0-8493-8558-X

  •  ISBN-13 978-0-8493-8558-2

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publication info   P. Brousseau, ed., CRC Press, 1998, 160 pp., hard cover
mfr. no.   CRC Press

Description

General description

The Manual of Immunological Methods presents validated assays that are accompanied by a series of tools that will guide readers in performing their own laboratory work. It covers specific methodologies including sample collection, preparation of cells, assay conditions, and data evaluation. Discussion of species differences and comparisons of a wide variety of species is an important feature. Both graduate students and practicing researchers in the areas of immunology and immunotoxicology will appreciate the Manual of Immunological Methods as a guide to standard operating procedures in the laboratory.

Table of Contents

Identification, Anaesthesia, and Euthanasia
Identification of Laboratory Rodents by Ear Notching
Identification of Fish by Fin Clipping
Anaesthesia of Fish and Rodents
Euthanasia of Rodents
Euthanasia of Fish
Working Sheet: Weight of Laboratory Animals
Collection of Peripheral Blood Samples
Reagents
Materials and Equipment
Procedure
Removal of Organs
Reagents
Materials and Equipment
Procedure
Preparation of Cell Suspensions
Extrusion of Coelomocytes in the Earthworm
Hemocyte Collection in the Mollusk
Cell Suspension from Peritoneal Exudate
Isolation of White Blood
Cells from Peripheral Blood
Cell Suspensions from Lymphoid Organs
Assessment of Cell Viability
Determination of Cell Viability and Concentration by Trypan Blue Dye Exclusion
Determination of Cell Viability and Cell Concentration with Ethidium Bromide and Acridine Orange
Flow Cytometric Assessment of Cell Viability
Working Sheet: Cell Viability and Concentration by Trypan Blue Dye Exclusion or Ethidium Bromide/Acridine Orange
Cell Cryopreservation
Reagents
Materials and Equipment
Procedure
Special Recommendations
Analysis of Results
Species-Related Changes in the Protocol
Technique-Related Changes in the Protocol: Phagocytosis with Yeast Statistical Tests
Working Sheet: Phagocytic Function Oxidative Burst Assay Using Flow Cytometry
Reagents
Materials and Equipment Procedure
Special Recommendations
Analysis of Results
Species-Related Changes in the Protocol Statistical Tests
Working Sheet: Acquisition List Cell Cytotoxicity Natural Killer (NK) Cell Activity Antibody-Dependent Cellular Cytotoxicity Lymphokine-Activated Killer (LAK)
Working Sheet: Acquistion List Lymphoblastic Transformation
Reagents Materials and Equipment Procedure
Analysis of Result Special Recommendations
Species-Related Changes in the Protocol
Working Sheet: Mitogenic Assay Determination of Antibody-Producing Cells to a Specific Antigen Liquid Plaque Forming Cell Assay in Mouse Model Agar Plaque Forming Cell Assay in Rat Model
Working Sheet: Plaque Forming Cells (PFC) Mixed Lymphocyte Reaction (MLR)
Reagents Materials and Equipment Procedure Analysis of Results Special Recommendations
Species-Related Changes in the Protocol
Working Sheet: Mixed Lymphocyte Reaction Intracellular Levels of Calcium Assay Using Flow Cytometry
Reagents Materials and Equipment Procedure
Special Recommendations Analysis of Results
Species-Related Changes in the Protocol Statistical Tests
Working Sheet: Acquisition List Phenotyping of Blood Mononuclear Cells
Reagents Materials and Equipment Procedure
Special Recommendations Analysis of Results
Species-Related Changes in the Protocol Immunophenotype of Rat Peripheral Blood Lymphocytes
Working Sheet Evaluation of Intracellular Level of Thiols
Reagents Materials and Equipment Procedure
Special Recommendations Analysis of Results
Species-Related Changes in the Protocol
Working Sheet: Acquisition List Apoptosis
Reagents Materials and Equipment Procedure Analysis of Results

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