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M0393 Sigma

Medium 199

With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Properties

Related Categories Cell Culture, Classic Media and Salts, Medium 199, Powder Media
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   L-glutamine: yes
Hanks’ salts (2% CO2): yes
HEPES: no
sodium pyruvate: no
NaHCO3: no
phenol red: yes
storage temp.   2-8°C

Description

Application

Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.

Quantity

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Peer-Reviewed Papers
15

References

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