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M0535 Sigma

Mycoplasma Broth

powder, suitable for microbiology

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Properties

Related Categories Cell Culture, Mycoplasma Detection, Reagents and Supplements More...
form   powder
solubility   H2O: soluble25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)
suitability   suitable for microbiology

Description

Components

Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement.

Packaging

250, 500 g in poly bottle

Application

Mycoplasma Broth is used for the cultivation of mycoplasma. It was used to grow Porphyromonas gingivalis under anerobic conditions.

Recommended for the cultivation of mycoplasma.

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Biochem/physiol Actions

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth of the organism.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Mycoplasma Detection/Elimination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest concerns are mycoplasma contamination. Although mycoplasma do not usually kill contaminated cel...
Don Finley
BioFiles v7 n1, 2011, 14–15
Keywords: Eliminations, Metabolism, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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H1270 Horse Serum, Donor herd, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

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