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Molecular Fluorescence: Principles and Applications

  •  ISBN-10 3-527-29919-X

  •  ISBN-13 978-3-527-29919-5

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publication info   B. Valeur, Wiley-VCH, 2002, 402 pp., hard cover
mfr. no.   Wiley

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General description

Fluorescence spectroscopy is an important tool of investigation in many areas. Its advantage is extremely high sensitivity and selectivity - even single molecules can be detected - and it achieves a high spatial resolution and time resolution in combination with microscopic techniques or laser techniques, respectively. In biochemistry and molecular genetics, fluorescence spectroscopy has become a dominating technique. Together with the latest imaging techniques, fluorescence spectroscopy allows a real-time observation of the dynamics of intact biological systems with an unprecedented resolution.

Table of Contents

Introduction
Absorption of UV-visible light
Characteristics of Fluorescence Emission
Effects of Photophysical or Photochemical Inter- or Intramolecular Processes on Fluorescence Emission
Fluorescence Polarization. Emission Anisotropy
Principles of steady-state and time-resolved Fluorometric Techniques
Effect of Polarity on Fluorescence Emission. Polarity Probes
Microviscosity, Fluidity, molecular mobility. Estimation by Means of Fluorescent Probes
Resonance Energy Transfer and Applications
Fluorescent Molecular Sensors of Ions and Molecules
Advanced Fluorometric Techniques

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