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M0650 Sigma

Medium 199

10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Classic Media and Salts,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
concentration   10 ×
impurities   endotoxin, tested
suitability   suitable for cell culture
components   sodium pyruvate: no
L-glutamine: no
NaHCO3: no
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
storage temp.   2-8°C

Description

Application

Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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