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M0769 Sigma

Minimum Essential Medium Eagle

AutoMod, with Earle′s salts, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

Synonym: MEM

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Properties

Related Categories Cell Culture, Classic Media and Salts, Minimum Essential Medium Eagle (MEM), Powder Media
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   NaHCO3: no
HEPES: no
L-glutamine: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
phenol red: yes
storage temp.   2-8°C

Description

General description

Modification of the original MEM formulation. Contains a succinate buffering system. See formulations table for specific information. Medium may be autoclaved.

Other Notes

Modified for autoclaving.

Quantity

Formulated to contain 9.4 grams of powder per liter of medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Cryostor Cryopreservation Video Protocol

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

Minimum Essential Medium Eagle - MEM Media

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain sub...
Keywords: Addition reactions, Adhesion, Cell culture, Cloning, Culture media, Eliminations, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

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