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M1018 Sigma

Minimum Essential Medium Eagle

With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym: MEM

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Properties

Related Categories Cell Culture, Classic Media and Salts, Minimum Essential Medium Eagle (MEM), Powder Media
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes
NaHCO3: no
HEPES: no
phenol red: yes
storage temp.   2-8°C

Description

General description

Modification of the original MEM formulation. Earle's salts have been replaced with Hanks' salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Quantity

Formulated to contain 10.7 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Protocols & Applications

Cryostor Cryopreservation Video Protocol

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

Minimum Essential Medium Eagle - MEM Media

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain sub...
Keywords: Addition reactions, Adhesion, Cell culture, Cloning, Culture media, Eliminations, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

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