|Related Categories||Biochemicals, Buffer / Buffer Salts, Buffers / Buffer Salts, Buffers / Buffer salts, Chemical Synthesis,|
|grade||for molecular biology|
|sterility||0.2 μm filtered|
|concentration||1.00 M±0.01 M|
|cation traces||heavy metals (as Pb): ≤5 ppm|
|foreign activity||DNase, RNase, NICKase and protease, none detected|
|storage temp.||room temp|
Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.
Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.
Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions.
1. OBJECTIVE To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Fructose -6- Phosphate Kinase (Pyrophosphate Dependent) from Propionibacterium Freudenreichii (Shermanii).
Keywords: Atomic layer deposition, Extinction coefficient
1. OBJECTIVE To standardize a procedure for the enzymatic determination of Glucose-6-Phosphate Dehydrogenase.
Keywords: Extinction coefficient
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia ,Sigma Product Number L9156, using a...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography
The Continuous Spectrophotometric Rate Determination (A600, Light path = 1 cm) is based on the following reaction:
Replaces OP SP-ADP01. New document assigned to conform to current document numbering system. Refer to CR SOP-DEK-ENZ47.
Keywords: Extinction coefficient, Molecular biology
Multiplex qPCR is a technique that simultaneously amplifies two or more target sequences in a single reaction. Analysis of multiple targets per sample allows for conservation of limited starting mate...
Jaime Miller, Deborah Vassar
This procedure may be used for all Phosphoglucomutase products except for β-Phosphoglucomutase, Catalog Number P4109.
Keywords: Enzyme activity, Extinction coefficient
Maternal magnesium supplementation reduces intrauterine growth restriction and suppresses inflammation in a rat model. Roman A, Desai N, Rochelson B, et al. Am. J. Obstet. Gynecol. 208(5), 383.e1-7, (2013)
Cation-dependent folding of 3' cap-independent translation elements facilitates interaction of a 17-nucleotide conserved sequence with eIF4G. Kraft JJ, Treder K, Peterson MS, et al. Nucleic Acids Res. 41(5), 3398-413, (2013)
Meta-analysis of randomized controlled trials on magnesium in addition to beta-blocker for prevention of postoperative atrial arrhythmias after coronary artery bypass grafting. Wu X, Wang C, Zhu J, et al. BMC Cardiovasc. Disord. 13, 5, (2013)
Rapid and efficient synthesis of nucleoside polyphosphates and their conjugates using sulfonyl imidazolium salts. Mohamady S and Taylor SD Curr. Protoc. Nucleic Acid Chem. Chapter 13, Unit13.11, (2012)
Differences in photosynthetic performance and its correlation with growth among tomato cultivars in response to different salts. Nebauer SG, Sánchez M, Martínez L, et al. Plant Physiol. Biochem. 63, 61-9, (2013)
Divalent cations enhance fluoride binding to Streptococcus mutans and Streptococcus sanguinis cells and subsequently inhibit bacterial acid production. Domon-Tawaraya H, Nakajo K, Washio J, et al. Caries Res. 47(2), 141-9, (2013)
Increased frequency and severity of developmental deformities in rough-skinned newt (Taricha granulosa) embryos exposed to road deicing salts (NaCl & MgCl2). Hopkins GR, French SS, and Brodie ED Environ. Pollut. 173, 264-9, (2013)
Effects of proteoliposome composition and draw solution types on separation performance of aquaporin-based proteoliposomes: implications for seawater desalination using aquaporin-based biomimetic membranes. Zhao Y, Vararattanavech A, Li X, et al. Environ. Sci. Technol. 47(3), 1496-503, (2013)
The nucleoid-associated protein Dan organizes chromosomal DNA through rigid nucleoprotein filament formation in E. coli during anoxia. Lim CJ, Lee SY, Teramoto J, et al. Nucleic Acids Res. 41(2), 746-53, (2013)
Acclimation of leaves to low light produces large grana: the origin of the predominant attractive force at work. Jia H, Liggins JR, and Chow WS Philos. Trans. R. Soc. Lond., B, Biol. Sci. 367(1608), 3494-502, (2012)
Concentration polarization phenomenon during the nanofiltration of multi-ionic solutions: influence of the filtrated solution and operating conditions. Déon S, Dutournié P, Fievet P, et al. Water Res. 47(7), 2260-72, (2013)
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