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M1028 Sigma

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

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Description

Components

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

General description

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It was used to prepare buffer to lyse cells for immunoprecipitation1 and to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.2

Price and Availability


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Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 
1

Protocols & Articles

Articles

Magnesium Chloride Solution for molecular biology

This product is a 1.00 ± 0.01 M solution, prepared in 18 megohm water and 0.2 µm filtered. It is designated as Molecular Biology grade and is suitable for molecular biology applications. This product...
Keywords: Enzymology, Molecular biology, Polymerase chain reaction, Sequencing

Protocols

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

1. OBJECTIVE To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE

Enzymatic Assay of Fructose-6-Phosphate Kinase Pyrophosphate Dependent

1. OBJECTIVE To standardize a procedure for the enzymatic assay of Fructose -6- Phosphate Kinase (Pyrophosphate Dependent) from Propionibacterium Freudenreichii (Shermanii).
Keywords: Atomic layer deposition, Extinction coefficient

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

1. OBJECTIVE To standardize a procedure for the enzymatic determination of Glucose-6-Phosphate Dehydrogenase.
Keywords: Extinction coefficient

Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

1. OBJECTIVE To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia ,Sigma Product Number L9156, using a...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products. The Continuous Spectrophotometric Rate Determination (A600, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of Myokinase (EC 2.7.4.3)

Replaces OP SP-ADP01. New document assigned to conform to current document numbering system. Refer to CR SOP-DEK-ENZ47.
Keywords: Extinction coefficient, Molecular biology

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Multiplex qPCR is a technique that simultaneously amplifies two or more target sequences in a single reaction. Analysis of multiple targets per sample allows for conservation of limited starting mate...
Keywords: AGE, Amplification, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Poisons, Polymerase chain reaction, Polymerase chain reaction - quantitative

Phosphoglucomutase Assay

This procedure may be used for all Phosphoglucomutase products except for β-Phosphoglucomutase, Catalog Number P4109.
Keywords: Extinction coefficient

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Analysis of androgen receptor rapid actions in cellular signaling pathways: receptor/Src association. Migliaccio A, Castoria G, and Auricchio F Methods Mol. Biol. 776, 361-70, (2011)

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2. Insulin-like growth factor-1 lowers spreading depression susceptibility and reduces oxidative stress. Grinberg YY, van Drongelen W, and Kraig RP J. Neurochem. 122(1), 221-9, (2012)

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Direct and real-time observation of rotary movement of a DNA nanomechanical device. Rajendran A, Endo M, Hidaka K, et al. J. Am. Chem. Soc. 135(3), 1117-23, (2013)

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Effects of proteoliposome composition and draw solution types on separation performance of aquaporin-based proteoliposomes: implications for seawater desalination using aquaporin-based biomimetic membranes. Zhao Y, Vararattanavech A, Li X, et al. Environ. Sci. Technol. 47(3), 1496-503, (2013)

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Directly probing the effects of ions on hydration forces at interfaces. Kilpatrick JI, Loh SH, and Jarvis SP J. Am. Chem. Soc. 135(7), 2628-34, (2013)

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The nucleoid-associated protein Dan organizes chromosomal DNA through rigid nucleoprotein filament formation in E. coli during anoxia. Lim CJ, Lee SY, Teramoto J, et al. Nucleic Acids Res. 41(2), 746-53, (2013)

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Cognitive changes after saline or plasmalyte infusion in healthy volunteers: a multiple blinded, randomized, cross-over trial. Story DA, Lees L, Weinberg L, et al. Anesthesiology 119(3), 569-75, (2013)

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Divalent cations enhance fluoride binding to Streptococcus mutans and Streptococcus sanguinis cells and subsequently inhibit bacterial acid production. Domon-Tawaraya H, Nakajo K, Washio J, et al. Caries Res. 47(2), 141-9, (2013)

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Acclimation of leaves to low light produces large grana: the origin of the predominant attractive force at work. Jia H, Liggins JR, and Chow WS Philos. Trans. R. Soc. Lond., B, Biol. Sci. 367(1608), 3494-502, (2012)

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A hint on pharmacological and toxicological differences between magnesium chloride and magnesium sulphate, or of scallops and men. Durlach J, Bara M, and Theophanides T Magnes. Res. 9(3), 217-9, (1996)

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A protocol for the 0.9% saline versus Plasma-Lyte 148 for intensive care fluid therapy (SPLIT) study. Reddy SK, Bailey MJ, Beasley RW, et al. Crit. Care Resusc. 16(4), 274-9, (2014)

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[The effectiveness of certain magnesium salts in nephrolithiasis caused by the use of sodium oxalate and celecoxib]. Spasov AA, Iezhitsa IN, Kharitonova MV, et al. Urologiia. (1), 29-34, (2013)

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Purification, optimization of assay, and stability studies of dextransucrase isolated from Weissella cibaria JAG8. Mohan Rao TJ and Goyal A Prep Biochem Biotechnol. 43(4), 329-41, (2013)

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Rapid and efficient synthesis of nucleoside polyphosphates and their conjugates using sulfonyl imidazolium salts. Mohamady S and Taylor SD Curr. Protoc. Nucleic Acid Chem. Chapter 13, Unit13.11, (2012)

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Observing a DNA polymerase choose right from wrong. Freudenthal BD, Beard WA, Shock DD, et al. Cell 154(1), 157-68, (2013)

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Concentration polarization phenomenon during the nanofiltration of multi-ionic solutions: influence of the filtrated solution and operating conditions. Déon S, Dutournié P, Fievet P, et al. Water Res. 47(7), 2260-72, (2013)

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Inflammatory bowel disease: MR- and SPECT/CT-based macrophage imaging for monitoring and evaluating disease activity in experimental mouse model--pilot study. Wu Y, Briley-Saebo K, Xie J, et al. Radiology 271(2), 400-7, (2014)

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Cation-dependent folding of 3' cap-independent translation elements facilitates interaction of a 17-nucleotide conserved sequence with eIF4G. Kraft JJ, Treder K, Peterson MS, et al. Nucleic Acids Res. 41(5), 3398-413, (2013)

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Characterising protein, salt and water interactions with combined vibrational spectroscopic techniques. Perisic N, Afseth NK, Ofstad R, et al. Food Chem. 138(1), 679-86, (2013)

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Manganese accumulation in bone following chronic exposure in rats: steady-state concentration and half-life in bone. O'Neal SL, Hong L, Fu S, et al. Toxicol. Lett. 229(1), 93-100, (2014)

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Squid have nociceptors that display widespread long-term sensitization and spontaneous activity after bodily injury. Crook RJ, Hanlon RT, and Walters ET J. Neurosci. 33(24), 10021-6, (2013)

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Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface. Dai B, Kang SG, Huynh T, et al. Proc. Natl. Acad. Sci. U. S. A. 110(21), 8543-8, (2013)

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Magnesium chloride or magnesium sulfate: a genuine question. Durlach J, Guiet-Bara A, Pagès N, et al. Magnes. Res. 18(3), 187-92, (2005)

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Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China. Qiu XX, Mou YZ, Zhao ML, et al. Int. J. Syst. Evol. Microbiol. 63(Pt 11), 3975-80, (2013)

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Effects of soybean protein composition and processing conditions on silken tofu properties. Yang A and James AT J. Sci. Food Agric. 93(12), 3065-71, (2013)

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Importance of magnesium chloride repletion after myocardial infarction. Sheehan J Am. J. Cardiol. 63(14), 35G-38G, (1989)

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Effects of NH4Cl and MgCl(2) on pretreatment and xylan hydrolysis of miscanthus straw. Kang KE, Park DH, and Jeong GT Carbohydr. Polym. 92(2), 1321-6, (2013)

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K(+) , Na(+) , and Mg(2+) on DNA translocation in silicon nitride nanopores. Uplinger J, Thomas B, Rollings R, et al. Electrophoresis 33(23), 3448-57, (2012)

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Oral magnesium supplementation improves the metabolic profile of metabolically obese, normal-weight individuals: a randomized double-blind placebo-controlled trial. Rodríguez-Moran M and Guerrero-Romero F Arch. Med. Res. 45(5), 388-93, (2014)

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Leishmania amazonensis: characterization of an ecto-pyrophosphatase activity. Freitas-Mesquita AL, Fonseca-de-Souza AL, and Meyer-Fernandes JR Exp. Parasitol. 137, 8-13, (2014)

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Increased frequency and severity of developmental deformities in rough-skinned newt (Taricha granulosa) embryos exposed to road deicing salts (NaCl & MgCl2). Hopkins GR, French SS, and Brodie ED Environ. Pollut. 173, 264-9, (2013)

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Maternal magnesium supplementation reduces intrauterine growth restriction and suppresses inflammation in a rat model. Roman A, Desai N, Rochelson B, et al. Am. J. Obstet. Gynecol. 208(5), 383.e1-7, (2013)

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Bacterial maximum non-inhibitory and minimum inhibitory concentrations of different water activity depressing solutes. Cebrián G, Arroyo C, Mañas P, et al. Int. J. Food Microbiol. 188, 67-74, (2014)

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Characterization of a variant of Xanthomonas citri subsp. citri that triggers a host-specific defense response. Chiesa MA, Siciliano MF, Ornella L, et al. Phytopathology 103(6), 555-64, (2013)

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Effect of salt reduction on growth of Listeria monocytogenes in meat and poultry systems. Harper NM and Getty KJ J. Food Sci. 77(12), M669-74, (2012)

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Differences in photosynthetic performance and its correlation with growth among tomato cultivars in response to different salts. Nebauer SG, Sánchez M, Martínez L, et al. Plant Physiol. Biochem. 63, 61-9, (2013)

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Common trace elements alleviate pain in an experimental mouse model. Tamba BI, Leon MM, and Petreus T J. Neurosci. Res. 91(4), 554-61, (2013)

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Structures of septin filaments prepared from rat brain and expressed in bacteria. Maimaitiyiming M, Kumanogoh H, Nakamura S, et al. Protein Expr. Purif. 87(2), 67-71, (2013)

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Meta-analysis of randomized controlled trials on magnesium in addition to beta-blocker for prevention of postoperative atrial arrhythmias after coronary artery bypass grafting. Wu X, Wang C, Zhu J, et al. BMC Cardiovasc. Disord. 13, 5, (2013)

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Elevated ferric, calcium and magnesium ions in the brain induce protein aggregation in brain mitochondria. Alleyne T, Mohan N, and Adogwa A West Indian Med. J. 61(2), 122-7, (2012)

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Magnesium supplementation reduces intrauterine growth restriction and inflammation: how and why? Das UN Am. J. Obstet. Gynecol. 209(5), 497-8, (2013)

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Reply: To PMID 23474429. Metz C, Roman A, Gupta M, et al. Am. J. Obstet. Gynecol. 209(5), 498-9, (2013)

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Heparin selectively affects the quantification of microRNAs in human blood samples. Boeckel JN, Thomé CE, Leistner D, et al. Clin. Chem. 59(7), 1125-7, (2013)

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Merck 14,5662

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