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M1028 Sigma

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

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Description

Components

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

General description

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It was used to prepare buffer to lyse cells for immunoprecipitation and to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Price and Availability


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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Magnesium Chloride Solution for molecular biology

This product is a 1.00 ± 0.01 M solution, prepared in 18 megohm water and 0.2 µm filtered. It is designated as Molecular Biology grade and is suitable for molecular biology applications. This product...
Keywords: Enzymology, Molecular biology, Polymerase chain reaction, Sequencing

Protocols

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE

Enzymatic Assay of Fructose-6-Phosphate Kinase Pyrophosphate Dependent

To standardize a procedure for the enzymatic assay of Fructose -6- Phosphate Kinase (Pyrophosphate Dependent) from Propionibacterium Freudenreichii (Shermanii).
Keywords: Atomic layer deposition, Extinction coefficient

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To standardize a procedure for the enzymatic determination of Glucose-6-Phosphate Dehydrogenase.
Keywords: Extinction coefficient

Enzymatic Assay of Hexokinase

This procedure applies to most products1 that have a specification for Hexokinase determination by enzymatic determination.
Keywords: Extinction coefficient

Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia, Sigma Product Number L9156, using a coupled enzy...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products. The Continuous Spectrophotometric Rate Determination (A600, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of Myokinase (EC 2.7.4.3)

Replaces OP SP-ADP01. New document assigned to conform to current document numbering system. Refer to CR SOP-DEK-ENZ47.
Keywords: Extinction coefficient, Molecular biology

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Multiplex qPCR is a technique that simultaneously amplifies two or more target sequences in a single reaction. Analysis of multiple targets per sample allows for conservation of limited starting mate...
Keywords: AGE, Amplification, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Poisons, Polymerase chain reaction, Polymerase chain reaction - quantitative

Phosphoglucomutase Assay

This procedure may be used for all Phosphoglucomutase products except for β-Phosphoglucomutase, Catalog Number P4109.
Keywords: Extinction coefficient

Peer-Reviewed Papers
15

References

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