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M1567 Sigma

Malic Dehydrogenase from porcine heart

≥600 units/mg protein (biuret), ammonium sulfate suspension

Synonym: L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
form   ammonium sulfate suspension
foreign activity   Glutamic-Oxalacetic Transaminase ≤0.01%
  Glutamic-Pyruvic Transaminase ≤0.01%
storage temp.   2-8°C

Description

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

Physical form

Suspension in 2.8 M (NH4)2SO4 solution, pH 6.0

Application

Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.

General description

Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Protocols

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

The continuous spectrophotometric rate determination (A340, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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