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  • M1570 - Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

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M1570 Sigma

Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

~1.0 mg/mL, clone MY-32, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   feline, rat, bovine, chicken, human, mouse, rabbit, guinea pig
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using porcine tongue
  microarray: suitable
  western blot: 0.5-1.0 μg/mL using total extract of rabbit skeletal muscle
clone   MY-32, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rabbit muscle myosin.

General description

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.

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