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M2003 Sigma

MTT Formazan

powder

Synonym: 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

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Properties

Related Categories Cell Biology, Hematology and Histology, M, Stains & Dyes, A to Z, Stains and Dyes More...
InChI Key   GIFKVQOPAJDJLO-SZVLODPRSA-N
assay   ≥97% (TLC)
form   powder
color   purple
solubility   acetone: soluble1 mg/mL

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 g in glass bottle

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Can MTT Formazan, Product M2003, be used in cell viability assays?
No.  MTT or thiazolyl blue tetrazolium is used to determine the number of viable cells in well-cultures. Consider use of kit CGD1 or product M5655.
What is the solubility of MTT Formazan, Product M2003, in water or PBS?
This product is not soluble in either water or PBS.  Sigma Aldrich tests product at 1mg/mL in acetone. A 1:1 DMSO:acidified isopropanol also works well for dissolving the formazan.
Can MTT Formazan, Product M2003, be used to measure dehydrogenases?
No. MTT is converted by active mitochondrial dehydrogenases of living cells to MTT Formazan. Consider use of product M2128 or M5655.
What is the proper wavelength for measuring MTT Formazan, Product M2003?
We recommend detection at 570 nm, with background subtraction at 630-690 nm in acidified isopropanol.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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