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M2128 Sigma

Thiazolyl Blue Tetrazolium Bromide

98%

Synonym: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide

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Properties

Related Categories Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Cell Biology, Cell Culture, Cell Viability, Core Bioreagents,
InChI Key   AZKSAVLVSZKNRD-UHFFFAOYSA-M
assay   98%
form   powder
mp   195 °C (dec.)(lit.)
solubility   H2O: soluble5 mg/mL
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5, 10 g in glass bottle

100, 250, 500 mg in poly bottle

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
XF8060000
Protocols & Articles

Articles

Cell Viability and Proliferation

Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
Mark Frei
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents

Protocols

Recycling Micro-Assay of β-NAD and β-NADH

1. OBJECTIVE To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH at Sigma-Aldrich Saint Louis.
Keywords: Sonication

Peer-Reviewed Papers
15

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