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  • M220 - Monoclonal Anti-Nicotinic Acetylcholine Receptor (α7 Subunit) antibody produced in mouse

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M220 Sigma

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α7 Subunit) antibody produced in mouse

~5 mg/mL, clone mAb 306, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Ion Channels,
species reactivity   human (denatured), rat (denatured), chicken
application(s)   immunohistochemistry (frozen sections): 1:3,000
clone   mAb 306, monoclonal
concentration   ~5 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CHRNA7(1139)
rat ... Chrna7(25302)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

native and denatured α7 subunit (amino acids 380-400, binds α-bungarotoxin) of the nicotinic acetylcholine receptor from chicken and the denatured α7 subunit from rat.

General description

Nicotinic acetylcholine receptors (nAChRs) function as ligand-gated channels that modulate endoplasmic reticulum stress and neurotransmitter release. These receptors also control synaptic signaling in ganglia and muscles. The α7 form of nAChR is expressed in mitochondria and regulates Ca2+ transport and the release of cytochrome c. When expressed in non-excitable cells, α7 nAChR mediates cell growth and survival
Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is specific for native and denatured α7 subunits in chick, as well as for the denatured α7 subunits in rats and humans. The antibody does not bind well to the native form of the mammalian α7 subunit. The product can be used in chick, rat and human tissues.

Physical form

Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.

Application

Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is suitable for use in immunocytochemistry (at 1:3000-1:50 dilutions using paraformaldehyde fixed, cultured rat chromaffin cells) , and immunohistochemistry (at 1:3,000 dilutions using frozen sections).

Legal Information

Sold with permission of the Salk Institute

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