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M2378 Sigma

Mucin from porcine stomach

Type II

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Properties

Related Categories Biochemicals and Reagents, Mucin, Proteins and Derivatives, Structural Proteins
type   Type II
composition   bound sialic acid, ≤1.2%
solubility   1 M NaOH: soluble20 mg/mL
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

100, 500 g in poly bottle

Application

Mucin from porcine stomach was used in studies on the binding site of the galactose-specific agglutinin PA-IL from Pseudomonas aeruginosa.

Biochem/physiol Actions

Mucus forms the protective cover for all epithelial surfaces. The major structural component of mucus is gel-forming mucins. The mucus layer covering the intestinal epithelium has the MUC2 mucin as its central molecule. MUC2 is produced by goblet cells.

General description

Mucins or mucus glycoproteins are the main macromolecular components of mammalian mucus. It was also used in a study to evaluate the sputum smears concentrated by cyto-centrifugation for detection of acid-fast bacilli.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What does the "Type" designation in Mucin from porcine stomach mean?
The type designation does not have any particular biological significance.  It is used to emphasize differences in the listings of this product.
What are the differences between Mucin from porcine stomach Products M2378 and M1778?
Product No. M2378 is a crude mucin preparation. Product No. M1778 is a partially purified powder prepared according to the method described in Glenister, D.A. and Salmon. K. Microbial Ecol. in Health & Disease 1, 31, (1988). The amount of bound sialic acids are also different. Please refer to the specification sheets on our website: http://www.sigmaaldrich.com/catalog/search/SpecificationSheetPage/SIGMA/M2378 http://www.sigmaaldrich.com/catalog/search/SpecificationSheetPage/SIGMA/M1778
Is Product M2378, Mucin from porcine stomach, a purified preparation of mucin?
No. This product is a crude preparation of mucin; it is a mixture of glycoproteins.  It is obtained by digestion of hog stomach with pepsin, followed by precipitation and other steps.
How can I solubilize Product M2378, Mucin from porcine stomach?
This crude mucin may not be readily soluble in solution. At 20 mg/mL in 1 M sodium hydroxide, turbid suspensions are observed. To try to increase the solubility, one might try further dilution and sonication, but we have not tried this in our labs.
What is the molecular weight of Product M2378, Mucin from porcine stomach?
This product is a crude mixture of glycoproteins. The sugar and amino acid composition of the mucin has not been determined.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
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References

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