Anti-Glutamate Receptor NMDAR2B (NR2B) antibody produced in rabbit
affinity isolated antibody, lyophilized powder
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MDL number MFCD01865097
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Properties
| Related Categories | Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Glutamate Receptors, |
| species reactivity | rat, mouse, human |
| application(s) | immunohistochemistry (frozen sections): 1:1,000 |
| immunoprecipitation: 3 μL using 200 μg of rat brain | |
| western blot: 1 μg/mL using 35S-Protein A (with ECL dilutions may be higher) | |
| clone | polyclonal |
| antibody form | affinity isolated antibody |
| form | lyophilized powder |
| mol wt | antigen mol wt 180 kDa |
| storage temp. | −20°C |
| Gene Information |
human ... GRIN2B(2904) mouse ... Grin2b(14812) rat ... Grin2b(24410) |
Description
Immunogen
C-terminal portion of NR2B (amino acids 984-1104).
General description
The functional N-Methyl-D-Aspartate (NMDA) receptor complex consists of a NMDAR1 (NR1) subunit and atleast one NMDAR2 subunit ranging from NR2A-NR2D. These receptor complexes have been implicated in cochlear functions and neuronal plasticity. The C-terminal end of NR2B receptor may regulate synaptic localization 1,2,3
Anti-Glutamate Receptor NMDAR2B (NR2B) antibody is specific for NR2B subunit in mice, rats and humans. This antibody does not react with NR2A or NR2C subunits. The labeling of NR2B subunit by immunoblotting is blocked by the preadsorption of the antibody with the immunogen.
Physical form
Lyophilized from ammonium bicarbonate (5 mM).
Application
Anti-Glutamate Receptor NMDAR2B (NR2B) antibody is suitable for use in immunohistochemistry (1:1,000 dilution, using frozen sections), immunoprecipitation (3 μL using 200 μg of rat brain), and indirect ELISA. The antibody may also be used for western blot (1 μg/mL using (35S)-Protein A) and immunoblot (180 kDa).
Price and Availability
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