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M3148 Sigma

2-Mercaptoethanol

for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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Properties

Related Categories Antioxidants and Reducing Agents for Protein Stabilization, Biochemicals and Reagents, Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting,
grade   for electrophoresis
  for molecular biology
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
InChI Key   DGVVWUTYPXICAM-UHFFFAOYSA-N
product line   BioReagent
assay   99% (GC/titration)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
solubility   H2O: soluble1 mL/mL
density   1.114 g/mL at 25 °C(lit.)
suitability   suitable for cell culture
foreign activity   DNase, RNase, protease, none detected
storage temp.   room temp

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Reducing agent

Packaging

25, 100, 250, 500 mL in glass bottle

Price and Availability

Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
154.4 °F
Flash Point(C) 
68 °C
Protocols & Articles

Protocols

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Peer-Reviewed Papers
15

References

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