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M3148 Sigma

2-Mercaptoethanol

for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

DOWNLOAD MSDS (PDF)

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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Properties

Related Categories Core Bioreagents, DNA & RNA Purification, General Reagents, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Nucleic Acid Purification,
grade   for electrophoresis
  for molecular biology
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
product line   BioReagent
assay   99% (GC/titration)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
density   1.114 g/mL at 25 °C(lit.)
suitability   suitable for cell culture
foreign activity   DNase, RNase, protease, none detected
storage temp.   room temp
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Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Reducing agent

Packaging

25, 100, 250, 500 mL in glass bottle

Price and Availability

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