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M3172 Sigma

Mitogen activated protein kinase from rat

≥95% (SDS-PAGE), buffered aqueous glycerol solution, recombinant, expressed in E. coli (N-terminal histidine tagged)

Synonym: ERK2, Extracellular-signal regulated kinase, MAP Kinase Activated from rat, MAPK



Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in E. coli (N-terminal histidine tagged)
assay   ≥95% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   ≥500 U/mg
mol wt   mol wt 42 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   rat ... Mapk1(116590)


Unit Definition

One unit of activated MAP kinase will transfer 1 nmole of phosphate from ATP to myelin basic protein per min at 30 °C.

Physical form

Solution in 20 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EGTA, 10% glycerol, 1 mM DTT, and 0.03% Brij®.

Biochem/physiol Actions

MAP kinase is a serine/threonine kinase that is activated by MEK through a phosphorylation cascade. The cascade starts with extracellular signals transmitted by growth factor-regulated receptor tyrosine kinases or G-protein coupled receptors. Dually phosphorylated (Thr and Tyr) MAP kinase translocates from the cytoplasm to the nucleus, where it phosphorylates protein transcription factors, transducing extracellular signals into genomic responses, proliferation or differentiation.

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