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M3682 Sigma

Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody produced in mouse

~2 mg/mL, clone ERK-PY193, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-pY-ERK

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, M1-MAR,
species reactivity   human, rat
application(s)   capture ELISA: suitable
  immunocytochemistry: suitable
  microarray: suitable
  western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol
clone   ERK-PY193, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen, ERK-1 mol wt 44 kDa
  antigen, ERK-2 mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop.

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) reacts specifically with the monophosphorylated tyrosine form of MAP kinase (ERK-1 and ERK-2, 44 kDa and 42 kDa, respectively). It does not recognize the non-phosphorylated, the doubly-phosphorylated, and the monophosphorylated threonine forms of the MAPK molecules, or the doubly-phosphorylated forms of JNK- or p38-MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.

Preparation Note

Prepared from a culture supernatant of bioreactor grown hybridoma.

Application

The antibody may be used in immunoblotting applications in whole cell extract of rat fibroblasts cell line, Rat1 at a working concentration of 1-5 μg/mL. It is suitable for immunocytochemistry, ELISA and protein microarray.

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