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M3795 Sigma

IgM, Kappa from murine myeloma

clone TEPC 183, purified immunoglobulin, buffered aqueous solution

Synonym: Mouse IgM-κ

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Properties

Related Categories Antibodies, Mouse Myeloma Proteins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
biological source   mouse
antibody form   purified immunoglobulin
clone   TEPC 183, monoclonal
assay   ≥90% (HPLC)
form   buffered aqueous solution
concentration   2.0-2.2 mg/mL (Determined by the absorbance at 280 nm (E1%/280 = 11.8). Each vial contains at least 1 mg of purified myeloma protein.)
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Physical form

Solution in 0.05 M Tris, 0.5 M sodium chloride, pH 8.0, containing 0.02% sodium azide

Application

IgM, κ from murine myeloma was used as standard in ELISA and dot-immunobinding assay in various studies.

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
IgM, κ from murine myeloma is produced by TEPC 183 tumor line introduced subcutaneously in BALB/c mice. It specifically recognizes mouse IgM. Identity and purity of the immunoglobulin is established by immunoelectrophoresis.

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Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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