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  • M3807 - Monoclonal Anti-MAP Kinase, Non-Phosphorylated ERK antibody produced in mouse

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M3807 Sigma

Monoclonal Anti-MAP Kinase, Non-Phosphorylated ERK antibody produced in mouse

~2 mg/mL, clone ERK-NP2, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Kinase/Phosphatase Biology,
species reactivity   rat, human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 5-25 μg/mL using rat brain extract
clone   ERK-NP2, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen, ERK-1 mol wt 44 kDa
  antigen, ERK-2 mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop.

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Monoclonal Anti-MAPK Kinase, Non-phosphorylated ERK specifically reacts with non-phosphorylated form of ERK-1 and ERK-2 (44 and 42 kDa, respectively). It weakly reacts with monophosphorylated peptides but not with double phosphorylated peptides of ERK. It does not react with either JNK or p38-MAPK.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody reacts specifically with the non-phosphorylated, non-activated form of MAP kinase (ERK-1 and ERK-2). Weak cross-reaction is observed with monophosphorylated (threonine or tyrosine), but not with diphosphorylated peptides of MAPK. The antibody does not recognize JNK or p38 MAPK. The epitope recognized by the antibody contains non-phosphorylated threonine 183 and tyrosine 185 which resides within the ERK-activation loop (e.g., amino acids 178-188 in ERK-2).

Preparation Note

Prepared from a culture supernatant of bioreactor grown hybridoma

Application

A working concentration of 5-25 μg/mL may be used for detection by immunoblotting in rat brain extract. The antibody has also been used at working dilution of 1:50 for MAPK quantification by flow cytometry in monocyte-derived macrophages (MDM) derived from Holstein cattle.

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