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M3900 Sigma

Murashige and Skoog Vitamin Solution

1000 ×, liquid, plant cell culture tested

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Properties

Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
concentration   1000 ×
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Components

Solution contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Vitamin powder (M3900) can be combined with Murashige and Skoog micro/macro-nutrients to generate basal media which when supplemented with organics, agar, auxins and cytokinins yield complete medium for growth plant tissue culture. The M3900 Solution is a 1000x concentrate which contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Protocols

Vitamin Mixtures - Plant Tissue Culture Protocol

Powdered vitamin mixtures are hygroscopic and must be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. The basic steps for preparing ...
Keywords: Filtration, Precipitation, Vitamins

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Peer-Reviewed Papers
15

References

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